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General description
Ubiquitin-conjugating enzyme E2 A (UniProt: P49459; also known as EC 2.3.2.23; E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A) is encoded by the UBE2A (also known as RAD6A) gene (GeneID: 7319) in human. UBE2A belongs to the ubiquitin-conjugating enzyme family and accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1, it plays a role in transcription regulation by catalyzing the mono-ubiquitination of histone H2B at Lys-120 that provides a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, and telomeric silencing. It is also a prerequisite for H3K4me and H3K79me formation. Defects in UBE2A gene have been linked to mental retardation, X-linked, syndromic, Nascimento-type mental retardation that is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior.
~17 kDa observed; 17.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Ubiquitin-conjugating enzyme E2 A (UBE2A).
Application
Anti-UBE2A Antibody, Cat. No. ABS2203, is a rabbit polyclonal antibody that detects UBE2A and has been tested for use Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A 1:500 dilution from a representative lot detected UBE2A in mouse kidney tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE2A in human pancreas, human cervical cancer, and human uterus tissues.
Biochem/physiol Actions
This rabbit polyclonal antibody detects Ubiquitin-conjugating enzyme E2 A (UBE2A) in HeLa cells and Mouse Kidney.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected UBE2A in HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| web metadata keyword.default | Anti-UBE2A antibody Sigma-Aldrich,HR6A antibody for western blot,UBE2A E2 ubiquitin-conjugating enzyme A antibody,UBE2A IgG primary antibody,UBE2A RAD6 homolog A antibody,UBE2A antibody for cell signaling studies,UBE2A antibody for immunofluorescence,UBE2A antibody for protein analysis,UBE2A antibody for protein-protein interaction studies,UBE2A antibody for research use,UBE2A antibody,UBE2A immunohistochemistry antibody paraffin,UBE2A protein antibody ab279578,high-purity UBE2A antibody,monoclonal UBE2A antibody ab31917 |
| NCBI accession no. | NP_003327 |
| UniProt accession no. | P49459 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... UBE2A(7319) |
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