- 商品介绍
- 规格参数
- 包装参数
General description
Product Source: E.coli
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human SCF is an 18.4 kDa protein containing 165 amino acid residues corresponding to the soluble secreted form of SCF.
Application
For most in vitro applications, SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL. Responding cells are (partial list): Stem cells, BM precursor cells.
Physical form
The sterile filtered solution was lyophilized from 10mM Acetic acid.
Preparation Note
The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be further diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. Reconstituted SCF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Analysis Note
Specific Activity: Chemicon′s human SCF is fully biologically active when compared to standards.The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2 ng/mL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in E. coli |
| assay | >98% (SDS-PAGE and HPLC) |
| specific activity | ≥5 x 10e5 U/mg |
| manufacturer/tradename | Chemicon® |
| impurities | <0.1 ng/μg Endotoxin (of SCF; 1EU/μg) |
| input | sample type hematopoietic stem cell(s) |
| NCBI accession no. | NM_000899.3,NM_003994.4 |
| UniProt accession no. | P21583 |
| shipped in | dry ice |
| storage temp. | -10 to -25°C |
| Gene Information | human ... KITLG(4254) |
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