- 商品介绍
- 规格参数
- 包装参数
General description
The thymus is considered as the primary lymphoid organ that is responsible for the generation and maturation of T cells. Thymic epithelial cells (TECs) account for the majority of thymic stromal components. These cells can be divided into cortical and medullary thymic epithelial cells based on their localization within the thymus. TECs have dual germ layer origins, with cortical cells arising from ectoderm and medullary cells arising from the endoderm. In an embryonic thymus, cortical cells are the dominant population, while medullary cells account for most of the TECs in the adult thymus. Cortical thymic epithelial cells are essential for the positive selection of T cells, whereas the medullary thymic epithelial cells (mTECS) play a role in inducing negative selection of highly reactive T cells that are required for establishing central self-tolerance. mTECs participate in self-tolerance by eliminating self-reactive T cells. Cortical cells that support positive selection also support post-selection maturation of single-positive thymocytes. They provide cytokines, such as IL-7 and SCF, to promote early thymic progenitors (ETP) cells and Delta-like ligand 4 (DLL4)-mediated Notch signaling to induce the differentiation of ETPs towards the T lineage. DLL4 is reported to be expressed at high levels in cortical cells, but not in medullary cells. Clone ER-TR4 reacts with epithelial-reticular cells in the cortex of murine thymus. (Ref.: Wang, HX., et al. (2020). Front. Immunol. 10; 3099; Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529; Cordier, AC., et al. (1980). Am. J Anat. 157(3); 227-263).
Immunogen
Thymic stromal cells isolated from C3H mice.
Application
Quality Control Testing
Evaluated by Immunohistochemistry in mouse thymus tissue sections.
Immunohistochemistry Applications: A 1:50 dilution of this antibody detected Cortical stromal cells in mouse thymus tissue sections.
Tested applications
Immunofluorescence Analysis: A representative lot detected Cortical stromal cells in Immunofluorescence applications (Tanaka, Y., et al., (2007). Blood. 110(1):107-15).
Immunohistochemistry Applications: A representative lot detected Cortical stromal cells in Immunohistochemistry applications (Van Vliet, E., et al., (1984). Eur J Immunol. 14(6):524-9; Scheijen, B., et al., (2004). J Biol Chem. 279(11):10476-83; Dooley, J., et al., (2006). J Immunol. 176(11):6484-90; Tanaka, Y., et al., (2007). Blood. 110(1):107-15; Moretti, F.A., et al., (2018). Elife. 7:e35816; Hollander, G.A., et al. (1995). Nature. 373(6512):350-3).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone ER-TR4 is a rat monoclonal antibody that detects cortical stromal cells.
Physical form
Purified rat monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 200 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | ER-TR4, monoclonal |
| purified by | affinity chromatography |
| species reactivity | mouse |
| packaging | antibody small pack of 100 μg |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable |
| isotype | IgMκ |
| epitope sequence | Unknown |
| Protein ID accession no. | N/A |
| UniProt accession no. | N/A |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
¥ 4164.19
-
询价
-
¥ 2814.26
-
¥ 1683.78
-
询价
-
询价
-
¥ 8293.41
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 14407.09
-
¥ 4404.38
-
¥ 2307.00
微信小程序
7X24小时在线咨询