- 商品介绍
- 规格参数
- 包装参数
General description
155 kDa
diabetes, insulin signaling, glucose
Immunogen
Peptide corresponding to the amino acid region encompassing the human, mouse, and rat phospho-Insulin Receptor (IR) Tyrosine 1158 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131.
Application
Detect phospho-IR/IGF1R (Tyr1158) using this Anti-phospho-IR/IGF1R (Tyr1158) Antibody validated for use in IC, IH & WB.
Immunoblot Analysis: A 1:1,000 dilution of this lot detected phospho-IR/IGF1R (Tyr1158) in RIPA lysate of untreated or insulin treated CHO cells transfected with IR.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
Biochem/physiol Actions
Human phospho-Insulin Receptor (IR) Tyrosine 1158 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131. Mouse and rat reactivity are expected based on 100% sequence homology.
Predicted to cross-react with mouse and rat based on 100% sequence homology, but have not been tested.
Physical form
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Affinity purified
Preparation Note
Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_000208.2,NM_001079817.1 |
| UniProt accession no. | P06213 |
| shipped in | dry ice |
| target post-translational modification | phosphorylation (pTyr1158) |
| Gene Information | human ... INSR(3643) |
| 长度(mm) | |
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