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General description
Synembryn-B (UniProt Q80XE1; also known as Protein Ric-8B, Resistance to inhibitors of cholinesterase 8B) is encoded by the Ric8b (also known as BC051080) gene (Gene ID 237422) in murine species. Ric-8A and Ric-8B stimulate nucleotide exchange of all Gα subunit classes by stabilizing the Gα nucleotide-free transition state. Ric-8A acts upon Gαi/q/13, while Ric-8B targets Gαs/olf. In addition to their function as guanine nucleotide exchange factors (GEFs), Ric proteins also act as molecular chaperones that directly participate in the posttranslational folding of nascent Gα subunits to promote G-protein biosynthesis and membrane association. Ric-8 knockdown reduces Gα steady-state abundances and overexpression of Ric-8 proteins enhances cellular Gα levels. In Ric-8A– or Ric-8B–delicient ES cells, newly produced Gα subunits are defective in the initial association with an internal membrane, resulting in a ∼10-fold faster turned over rate than in WT cells.
~63 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Recombinant protein corresponding to mouse Ric-8B/Synembryn-B.
Application
This Anti-Ric-8B/Synembryn-B Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Ric-8B/Synembryn-B.
Western Blotting Analysis: A representative lot detected Ric-8B protein in murine embryonic stem (mES) cells (mESCs) derived from wild-type (WT) and Ric-8A−/− mice, but not mESCs from Ric-8B−/− mice (Gabay, M., et al. (2011). Sci Signal.4(200):ra79).
Western Blotting Analysis: A representative lot detected the endogenous Ric-8B protein in rabbit reticulocyte lysates (RRL) as well as recombinant murine Ric-8B protein (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).
Immunoprecipitation Analysis: A representative lot immunoprecipitated the endogenous Ric-8B protein from rabbit reticulocyte lysates/RRL (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).
Biochem/physiol Actions
Expected to react with all murine and human spliced isoforms (UniProt Q80XE1 & Q9NVN3).
Analysis Note
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1:1,000 dilution of this antibody detected Ric-8B/Synembryn-B in 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rabbit, human |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_898995 |
| UniProt accession no. | Q80XE1 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RIC8B(55188) |
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