Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3,100UG,05-484,Sigma

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订货号 0BW0028
品牌型号 Sigma 05-484
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

175kDa


Immunogen

peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor


Application

Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors


Biochem/physiol Actions

The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.


Physical form

100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
Thiophilic adsoption and size exclusion chromatography


Preparation Note

2 years at -20°C


Analysis Note

routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells


Other Notes

Replaces: 04-337; 04-341


Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcemouse
Quality Segment100
antibody formpurified antibody
antibody product typeprimary antibodies
clone20G3, monoclonal
species reactivityhuman, mouse
manufacturer/tradenameUpstate®
technique(s)immunoprecipitation (IP): suitable,western blot: suitable
isotypeIgG1κ
NCBI accession no.NM_005228
UniProt accession no.P00533
shipped inwet ice
target post-translational modificationphosphorylation (non-phospho-Tyr1173)
Gene Informationhuman ... EGFR(1956)
mouse ... Egfr(13649)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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