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General description
Research area: APOPTOSIS
Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC
Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells
Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.
Biochem/physiol Actions
The Cdk7/cyclin H/Mat1 (CAK complex) plays a role in both cell cycle regulation and transcription. Within the complex, Cdk7 functions as the Cdk-activating kinase for cell cycle and as a part of the general transcription factor, Transcription Factor II H (TFIIH), for transcription process. Additionally, it also regulates gene expression by phosphorylating transcription factors such as estrogen receptor-α (ER), p53, retinoid receptors, androgen receptor, and estrogen receptor. Elevated expression levels of Cdk7, cyclin H, and MAT1 have been observed in breast cancer patients. Inhibition of cdk7 is associated with the development of colorectal cancer in humans.
Packaging
Also available in 250μg size, please inquire for pricing and availability.
Physical form
Ni2+/NTA-agarose
Preparation Note
1 year at -70°C
Analysis Note
routinely evaluated by phosphorylation of cdk7 substrate peptide
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| form | liquid |
| shelf life | 1 yr |
| mol wt | Mw 37.8 kDa (cyclin H),Mw 39.9 kDa (cdk7),Mw 62.8 kDa (MAT1) |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| solubility | soluble |
| NCBI accession no. | NM_001799.2 |
| UniProt accession no. | P50613 |
| storage temp. | −70°C |
| Gene Information | human ... CDK7(1022) |
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¥ 1573.49
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¥ 1302.89
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¥ 1866.29
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¥ 1653.62
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