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General description
Na(+)/H(+) exchange regulatory cofactor NHE-RF2 (NHERF-2) is also known as NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1 (SIP-1), Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2 (SLC9A3R2), and Tyrosine kinase activator protein 1 (TKA-1). NHERF-2 is a Scaffold protein that links ezrin/moesin/radixin family members to the actin cytoskeleton and regulates their surface expression. NHERF-2 is critical for the cAMP-mediated phosphorylation and inhibition of SLC9A3. NHERF-2 is also thought to act as scaffold protein in the nucleus.
~ 37 kDa observed. An uncharacterized band may appear at ~41 kDa in some lysates.
Immunogen
recombinant protein corresponding to human NHERF-2.
Application
Anti-NHERF-2 Antibody, clone 32B6 is a highly specific mouse monoclonal antibody, that targets NHERF2 & has been tested in western blotting, ICC & IP.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in A431 and NHERF-2 transfected HT1080 cells. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Batt cell lysate. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HaCaT keratinocytes cell lysate
Evaluated by Western Blotting in HaCaT keratinocytes cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected NHERF-2 in 10 µg of HaCaT keratinocytes cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 32B6, monoclonal |
| species reactivity | rat, human |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_001123484 |
| UniProt accession no. | Q15599 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SLC9A3R2(9351) |
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