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General description
Paired box protein Pax-3, also known as HuP2, and encoded by the gene name PAX3 or HUP2, is a transcription factor that may be involved in regulating cell proliferation, migration, and apoptosis. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma.
~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human PAX3 expressed in E. Coli
Application
Research Sub Category
Apoptosis - Additional
Detect PAX3 using this Anti-PAX3, clone 7D8G7 Antibody validated for use in western blotting & ELISA.
ELISA: A representative lot was used on control antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Apoptosis & Cancer
Physical form
Mouse monoclonal IgG1 ascitic fluid with up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in A549, A431, Jurkat, rat spleen, and mouse liver lysates.
Western Blotting Analysis: A 1:500-1:2,000 dilution of this antibody detected PAX3 in A549, A431, Jurkat, rat spleen, and mouse liver lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 7D8G7, monoclonal |
| species reactivity | human, rat, mouse |
| technique(s) | ELISA: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P23760 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PAX3(5077) |
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