- 商品介绍
- 规格参数
- 包装参数
General description
Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.
~200 kDa
Immunogen
Laminin isolated from EHS mouse sarcoma.
Epitope: Near cross region
Application
Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.
Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Biochem/physiol Actions
Other species have not been tested.
Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.
Physical form
Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Analysis Note
Evaluated on a representative lot by Western blot on purified mouse laminin-1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | AL-2, monoclonal |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunofluorescence: suitable,immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NM_005559.2 |
| UniProt accession no. | P25391,P55268 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Lama1(16772) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 12156.68
-
¥ 2707.32
-
¥ 4164.19
-
询价
-
询价
-
询价
-
询价
-
¥ 4039.86
-
¥ 8293.41
-
询价
-
询价
-
询价
-
¥ 1956.41
-
询价
-
询价
-
询价
-
¥ 15214.65
-
询价
-
¥ 1509.00
-
¥ 1586.69
微信小程序
7X24小时在线咨询