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General description
3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. The PhotoCol-RUT, Methacrylated Collagen Hydrogel Kit (CC322: CC322-1, CC322-2) is based upon purified type I bovine collagen methacrylate (ColMA), which when photocrosslinked provides a native-like 3D environment for cells. In addition to type I bovine collagen methacrylate, the kit includes the photoinitiator ruthenium/sodium persulfate and other reagents for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen that has been modified by reacting with the protein′s free amines, primarily the ɛ-amines groups of the lysine residues as well as the α-amines groups on the N-termini. >20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content that was isolated from a closed herd and purified using controlled manufacturing processes.PhotoCol is a registered trademark of Advanced BioMatrix, Inc.
Application
Photocrosslinking is initiated using a 400-450 nm light source.
Packaging
1 Kit
Analysis Note
Sterility (USP modified): No GrowthEndotoxin LAL: ≤ 10.0 EU/mlGel Formation: ≤ 40 minKinetic Gel Test: ≤ 40 minPurity (SDS Page Silver Stain): ≥ 99%Degree of Methacrylation: ≥ 20%pH: 3.0-4.0Osmolality: ≤ 40 mOsm/Kg H20Young′s Modulus: CharacteristicElectrophoretic Pattern: Characteristic
Other Notes
CC322-1 (Store at Room Temp): Neutralization Solution, 1 X 10 mL (CS226408), Ruthenium Photoinitiator, 1 X 100 mg (CS226407), Sodium Persulfate Photoinitiator, 2 X 500 mg (CS226406).
Note: The complete PhotoCol-RUT, Methacrylated Collagen Hydrogel Kit (CC322) consists of CC322-1 and CC322-2 sold seperately.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| application(s) | cell analysis |
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