- 商品介绍
- 规格参数
- 包装参数
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
~17 kDa observed.
Immunogen
Epitope: H3K4me2
KLH-conjugated synthetic peptide (ART[Kme2]QTAR-GC) corresponding to amino acids 1-8 of human histone H3.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
The unconjugated antiserum (Cat. No. 07-030) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, immunocytochemistry, immunofluorescence, and Western blotting applications.
This Anti-dimethyl Histone H3 (Lys4) Antibody, Alexa Fluor™ 555 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.
Biochem/physiol Actions
Broad species cross-reactivity is expected.
Specificity of this polyclonal antibody toward histone H3 Lys4 dimethylation was demonstrated by dot blot analysis using the unconjugated antiserum (Cat. No. 07-030).
Physical form
Protein A purified.
Purified rabbit polyclonal antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys4 dimethylation in A431 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 555 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, Tetrahymena sp. |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NP_003484.1 |
| UniProt accession no. | Q16695 |
| target post-translational modification | dimethylation (Lys4) |
| Gene Information | human ... HIST1H3F(8968) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 2290.21
-
询价
-
¥ 1451.54
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 3442.25
-
询价
-
询价
-
询价
-
¥ 4968.78
-
¥ 3290.05
-
¥ 4390.38
-
¥ 10277.12
-
¥ 2307.00
-
¥ 5061.38
-
¥ 2132.12
-
询价
微信小程序
7X24小时在线咨询