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General description
Research area: Cell Signaling
Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R, and untagged, recombinant, human p85α full length. The H1047R substitution is a somatic mutation in p110α that has been associated with tumours of the colon, stomach, breast, and brain. PI3Ks are categorized into three classes, namely class I, II, and III, based on their structural characteristics, substrate specificities, and the nature of lipid end-products. Class IA PI3Ks are composed of heterodimers that include a p110 catalytic subunit and a p85 regulatory subunit. In mammals, PIK3CA, PIK3CB, and PIK3CD, encode the p110 catalytic isoforms: p110α, p110β, and p110δ, respectively.
PI3Kα/p85α
Biochem/physiol Actions
PI3 kinases play a critical role in various cellular processes such as cell survival, proliferation, differentiation, apoptosis, DNA repair, angiogenesis, cellular metabolism, and motility. PI3Ks can activate the serine/threonine kinase AKT and other downstream effector pathways by transmitting signals from growth factors and cytokines into intracellular messages. Genetic mutation in p110α is observed in breast, pancreatic, colon, liver, and lung cancers.Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and can induce oncogenic transformation.
Packaging
Also available in 10 μg size and in bulk
Also available in 250μg size --call for pricing and availability and reference catalog number 14-792M when ordering the 250μg size.
Physical form
50 mM Tris/HCl pH8.0, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Level | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| product line | Upstate® |
| form | liquid |
| specific activity | (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.) |
| mol wt | Mw 125 kDa (p110α(H1047R)),Mw 84 kDa (p85α) |
| purified by | (Ni2+/NTA-agarose) |
| species reactivity | human |
| manufacturer/tradename | Upstate® |
| color | colorless |
| solubility | water: soluble |
| accession no. | U79143 |
| UniProt accession no. | P26450 (mouse),P27986,P42336 / P27986;P42337 / P26450 (mouse),P42336,P42337 (mouse),U79143 |
| application(s) | life science and biopharma |
| Gene Information | human ... PIK3CA(5290) |
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