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General description
Glycerol-3-phosphate acyltransferase 4 (UniProt: Q86UL3; also known as EC:2.3.1.15, 1-acylglycerol-3-phosphate O-acyltransferase 6, 1-AGP acyltransferase 6; 1-AGPAT 6, Acyl-CoA:glycerol-3-phosphate acyltransferase 4, Lysophosphatidic acid acyltransferase zeta, LPAAT-zeta, Testis spermatogenesis apoptosis-related protein 7, TSARG7) is encoded by the GPAT4 (also known as AGPAT6, TSARG7, UNQ551/PRO1108) gene (Gene ID: 137954) in human. GPAT4 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family that plays a role in triglyceride biosynthesis in several tissues. It is a rate-limiting enzyme that converts glycerol-3-phosphate (G3P) to 1-acyl-sn-glycerol-3-phosphate by incorporating an acyl moiety at the sn-1 position of the glycerol backbone. It is shown to be active against both saturated and unsaturated long-chain fatty acyl-CoAs. Its HXXXXD motif (aa 248-253) is reported to be essential for its acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. By increasing triglyceride synthesis, it protects cells against lipotoxicity induced by high levels of saturated fatty acids. (Ref.: Piccolis, M., et al. (2019). Mol. Cell. 74(1); 32-44; Chen, YQ., et al. (2008). J. Biol. Chem. 283(15); 10048-10057).
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-termina half of human Glycerol-3-phosphate acyltransferase 4 (GPAT4).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Human GPAT4 protein in HeLa cell lysate.
Tested Applications
Immunoprecipitation Analysis: A representative lot Immunoprecipitated Human GPAT4 protein in Immunoprecipitation applications (Zhu, X.G., et al. (2019). Mol Cell. 74(1):45-58).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
This rabbit polyclonal antibody detects Glycerol-3-phosphate acyltransferase 4 (GPAT4). It targets an epitope within 25 amino acids from the N-terminal half.
Physical form
Purified rabbit polyclonal antibody in PBS with 0.1% sodium azide.
Preparation Note
0.35 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| mol wt | calculated mol wt 52.07 kDa,observed mol wt ~46 kDa |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey, canine, rat, porcine, bovine, feline, horse, mouse |
| packaging | antibody small pack of 100 μL |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_848934 |
| UniProt accession no. | Q86UL3 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... GPAT4(100127206) |
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