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General description
Histone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribut to the chromatin structure in both prokaryotic and eukaryotic cells. Consecutive methylation of the histone H4 Lys20 (H4K20) is dependent on nucleosome age, and different H4K20 methylation levels are found at distinct nuclear loci. H4K20 monomethylation (H4K20me1) is a repressive mark of transcribed genes, whereas H4K20 trimethylation (H4K20me3) is found in repetitive elements in the human genome. More than 80% of H4K20 is dimethylated in human cells. At least three different methyltransferases (KMTs) have been identified that can catalyze H4K20 methylation, while only lysine demethylases (KDMs) for H4K20me1, but not for H4K20me2 or H4K20me3, have been discovered. The monomethyltransferase gene Setd8 knockout in mouse and in Drosophila is lethal due to defects in cell cycle progression.
~10 kDa observed.
Immunogen
Epitope: H4K20me1.
KLH-conjugated, branched synthetic peptide corresponding to amino acids 15-25 (AKRHR[Kme]VLRDN) of human Histone H4 with monomethylated Lys20.
Application
Anti-monomethyl Histone H4 (Lys20), Alexa Fluor™ 555 conjugate is an antibody against monomethyl Histone H4 (Lys20), Alexa Fluor
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
The unconjugated antibody (Cat. No. 07-748) is shown to be suitable also for Immunocytochemistry, Multiplex assay, and Western blotting applications.
Biochem/physiol Actions
Broad species cross-reactivity expected due to sequence homology.
This polyclonal antibody detects histone H4 with monomethylated Lys20 (H4K20me1).
Physical form
Protein A purified
Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H4 Lys20 monomethylation in HeLa cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 555 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NM_001034077.4 |
| UniProt accession no. | P62805 |
| target post-translational modification | monomethylation (Lys20) |
| Gene Information | human ... HIST2H4B(554313) |
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