- 商品介绍
- 规格参数
- 包装参数
General description
Also known as ARD1 (Activator of RNA decay), NIPP-1 (nuclear inhibitor of protein phosphatase 1) is a ubiquitously expressed multifunctional nuclear scaffold protein of 39 kDa, which plays an important role in integrating transcription, RNA processing, and cell cycle progression. NIPP-1 serves as a signaling hub in the nucleus. Additionally, NIPP-1 contains at least two binding sites for PP1 and is complexed to about one-third of the nuclear pool of PP1. Normally, the NIPP-1 - PP1 complex is inactive, but can be activated following phosphorylation of NIPP-1 with PKA, CFK2, or Src family kinases. NIPP-1 also interacts directly with nucleic acids and components of polycomb repressive complexe 2 (PRC2) and functions as a PRC2-dependent transcriptional repressor.
~42 kDa observed. Three isoforms at 39 kDa (alpha isoform), 23 kDa (beta isoform), and 13 kDa (gamma isoform) are known to exist. An uncharacterized band may be observed at ~62 kDa in some nuclear extracts.
Immunogen
GST-tagged recombinant protein corresponding to human NIPP-1.
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NIPP-1 in Raji cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
This NIPP-1 antibody is validated for use in WB & ICC for the detection of the NIPP-1 protein.
Biochem/physiol Actions
This antibody should recognize the alpha and beta isoforms of NIPP-1.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Raji nuclear extract
Evaluated by Western Blot Analysis in Raji nuclear extract.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected NIPP-1 in 10 µg of Raji nuclear extract.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 300 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_002704 |
| UniProt accession no. | Q12972 |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP1R8(5511) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
¥ 2622.09
-
¥ 1683.78
-
¥ 1573.49
-
¥ 9207.92
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 9737.84
-
询价
-
¥ 238.17
-
询价
-
询价
-
询价
-
询价
-
¥ 4968.78
微信小程序
7X24小时在线咨询