Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)，100UG，OP140-100UGCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).

Immunogen

Epitope: within amino acids 37-45 of p53
recombinant, human p53 expressed in E. coli

Application

Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue

Other Notes

Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	mouse
Quality Level	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	DO-7, monoclonal
form	liquid
does not contain	preservative
species reactivity	bovine, monkey, human
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze,avoid repeated freeze/thaw cycles
dilution	(Frozen Sections (1-2 µg/mL) Immunoblotting (1-2 µg/mL) Immunofluorescence (0.5 µg/mL) Immunoprecipitation (2 µg/mg lysate) Paraffin Sections (1-2 µg/mL))
isotype	IgG2b
shipped in	wet ice
storage temp.	−20°C
target post-translational modification	unmodified
Gene Information	human ... TP53(7157)

长度(mm)
宽度(mm)
高度(mm)
重量(kg)