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General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2A.X is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition, this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins at a damaged site.
~16 kDa observed
Immunogen
Epitope: a.a. 121-139
KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.X.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-Histone H2A.X Antibody, Human (Rabbit Polyclonal Antibody) validated in WB to detect Histone H2A.X also known as H2AFX.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected 500 ng of recombinant Histone H2A.X.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of Rat2 acid extract, BALB 3T3/A31 acid extract, and calf thymus histone extract tissue lysate.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Histone H2A.X in 1X10E6 HeLa cells.
Physical form
Peptide affinity chromatography
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Analysis Note
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of HeLa acid extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human, rat, bovine |
| manufacturer/tradename | Chemicon®,Upstate® |
| technique(s) | flow cytometry: suitable,western blot: suitable |
| NCBI accession no. | NP_002096 |
| UniProt accession no. | P16104 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | bovine ... H2Ax(531733) human ... H2AX(3014) mouse ... H2Ax(15270) rat ... H2Ax(500987) |
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