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General description
72 kDa
Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr31 and Tyr118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.
Immunogen
Clone M102 was generated from phospho-Paxillin (Tyr31) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 31 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
Application
Detect phospho-Paxillin (Tyr31) using this Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 validated for use in WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Biochem/physiol Actions
The antibody detects Paxillin phosphorylated at Tyr31.
Physical form
Format: Purified
Protein A chromatography
Purified monoclonal IgG1 in phosphate-buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
Preparation Note
Maintain at -20°C for one year from date of receipt. Do not aliquot.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Pervanadate treated L6 cell lysates
Evaluated by western Blot in pervanadate treated L6 cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 µg of pervanadate treated L6 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB1146
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | M102, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P49023 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pTyr319) |
| Gene Information | human ... PXN(5829) |
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