Anti-PI3 Kinase Antibody, p85，200UL，ABS234，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Phosphatidylinositol 3-Kinase (PI3 Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β,γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibited by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
~85 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to rat PI3 Kinase, p85.

Application

Detect PI3 Kinase, p85 using this rabbit polyclonal antibody, Anti-PI3 Kinase Antibody, p85 validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot immunoprecipitated PI3 Kinase, p85 in 0.5 mg of Jurkat cell lysate.

Analysis Note

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:600 dilution of this antibody detected PI3 Kinase, p85 in 10 µg of Jurkat cell lysate.

Other Notes

Replaces: 06-497

技术参数 Specifications

biological source	rabbit
Quality Segment	100
conjugate	unconjugated
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	rat, human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)	immunoprecipitation (IP): suitable,western blot: suitable
NCBI accession no.	NP_037137
UniProt accession no.	Q63787
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	bovine ... Pik3R1(282307) human ... PIK3R1(5295) mouse ... Pik3R1(18708) rat ... Pik3R1(25513) rhesus monkey ... Pik3R1(698996)

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