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General description
Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: Q63787; also known as PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha) is encoded by the Pik3r1 gene (Gene ID: 25513) in rat. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding to receptor and activation of receptor tyrosine kinase, the p85/p110 complex is recruited to the receptor by interaction of the SH2 domain of p85 with consensus phosphotyrosine residues on receptor tyrosine kinase. This allows the p110 catalytic subunit to come in close proximity to its lipid substrates in the cell membrane. The interaction of a receptor tyrosine kinase with the p85 subunit relieves the inhibitory effect of p85 on the p110 catalytic subunit. Activated PI 3-kinase phosphorylates phosphoinositol (PI) substrates to produce PI(3)P, PI(3,4)P2, and PI(3,4,5)P3 (PIP3). These molecules act as second messengers and recruit PI 3-kinase-dependent serine/threonine kinases (PDK1) and Akt from the cytoplasm to the plasma membrane. PI 3-kinase activity is tightly regulated in normal cells. Over-activation of this pathway can perturb the control mechanisms for cell growth and survival and contribute to metastatic competence and resistance to chemotherapy. Abnormal activation of PI 3-kinases is seen in several forms of cancer. About 30% of solid tumors contain mutations in the catalytic unit of their PI 3-kinase. PI3 kinase plays a critical role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in Pik3r1 gene are known to be associated with insulin resistance.
~80 kDa observed; 83.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of p85 regulatory subunit of PI-3 kinase alpha.
Application
Detect PI 3-kinase alpha, p85 subunit using this rabbit polyclonal Anti-PI3 Kinase, p85, Cat. No. ABS1856 that has been tested in Immunoprecipitation and Western Blotting.
Immunoprecipitation Analysis: 2.5 µg from a representative lot detected PI3-Kinase, p85 in jurkat cell lysate.
Research Category
Signaling
Biochem/physiol Actions
This polyclonal antibody detects p85 regulatory subunit of PI3 kinase in multiple species. It targets an epitope within 18 amino acids from the C-terminal region.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PI3-Kinase, p85 in 10 µg of Raji cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: 06-195
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), mouse (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_037137 |
| UniProt accession no. | Q63787 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... PIK3R1(5295) |
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¥ 2013.69
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¥ 1779.22
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