Anti-CD64 Mouse mAb (10.1)，100UG，217620-100UGCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.
Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.
Recognizes the ~72 kDa CD64 protein (FcR1 receptor).

Immunogen

Human
human rheumatoid synovial fluid cells/monocytes

Application

Flow Cytometry (10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Analysis Note

Negative Control
HPB-MLT
Positive Control
4937 cells

Other Notes

Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453.
The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x10⁵ cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	mouse
Quality Segment	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	10.1, monoclonal
assay	≥95% (SDS-PAGE)
form	liquid
contains	0.5 mg/mL gentamycin sulfate as preservative
species reactivity	human
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze
dilution	(Flow Cytometry (10 µg/mL) Frozen Sections Immunoblotting Immunoprecipitation Neutralization Studies )
isotype	IgG1
shipped in	wet ice
storage temp.	2-8°C
target post-translational modification	unmodified

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