- 商品介绍
- 规格参数
- 包装参数
General description
57.00 kDa calculated.
Cyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt: Q96BA8; also known as cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS) is encoded by the CREB3L1 (also known as OASIS, PSEC0238) gene (Gene ID: 90993) in human. OASIS is a single-pass type II membrane protein that serves as a transcription factor and is involved in
unfolded protein response (UPR). It binds the DNA consensus sequence 5′-GTGXGCXGC-3′. It has a cytoplasmic domain (aa 1-374), a helical domain (aa 375-395), and a luminal domain (aa 396-519). In the absence of endoplasmic reticulum (ER) stress, it is inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. However, in response to ER stress, it is transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. OASIS also plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. It directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. OASIS is also required to protect astrocytes from ER stress-induced cell death. In astrocytes, it binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation. Mutations in CREB3L1 gene are reported to cause osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma.
Immunogen
KLH-conjugated linear Peptide corresponding to 35 amino acids from the N-terminal region of human OASIS (CREB3L1).
Application
Anti-OASIS (CREB3L1), clone 10H1, Cat. No. MABE1151, is a highly specific mouse monoclonal antibody that targets Cyclic AMP-responsive element-binding protein 3-like protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected OASIS (CREB3L1) in human pancreas and human tonsil tissues.
Immunohistochemistry Analysis: A representative lot detected OASIS (CREB3L1) in Immunohistochemistry applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233).
Western Blotting Analysis: A representative lot detected OASIS (CREB3L1) in Western Blotting applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233; Chen, Q., et. al. (2016). Mol Cell. 63(4):567-78).
Biochem/physiol Actions
Clone 10H1 detects Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) in human tissues. it targets an epitope within 35 amino acids from the N-terminal region.
Physical form
Format: Purified
Analysis Note
Evaluated by Immunohistochemistry in human prostate cancer tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected OASIS (CREB3L1) in human prostate cancer tissue sections.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 10H1, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_443086 |
| UniProt accession no. | Q96BA8 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... CREB3L1(90993) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
¥ 12156.68
-
¥ 2528.88
-
¥ 2814.26
-
¥ 1259.40
-
¥ 1563.36
-
询价
-
¥ 2013.69
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 8982.64
-
¥ 1568.60
-
¥ 2666.57
-
¥ 2617.87
-
¥ 1749.15
-
¥ 2132.12
-
¥ 954.17
微信小程序
7X24小时在线咨询