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General description
Protein phosphatase 1 regulatory subunit 12A (UniProt: Q90623; also known as 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, PP1M subunit M110, Protein phosphatase myosin-binding subunit, MYPT1) is encoded by the PPP1R12A (also known as MBS, MYPT1) gene (Gene ID: 396020) in human. MYPT1 is one of the subunits and an integral component of the Myosin phosphatase. It is detected in brain, lung, aorta, heart, gizzard, stomach, oviduct, spleen, kidney, and small intestine. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity. It is phosphorylated by Rho-associated kinases on serine and threonine residues. Phosphorylation at Threonine 695 is shown to inhibit myosin phosphatase activity and phosphorylation at Threonine 850 is reported to abolish myosin binding. Two isoforms of MYPT1 have been reported that are produced by alternative splicing.
~120/130 kDa observed; 111.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of chicken MYPT1.
Application
Detect MYPT1 using this rabbit polyclonal antibody, Anti-MYPT1 Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: 0.5µg from a representative lot immunoprecipitated MYPT1 from 0.5mg of HeLa cell lysate.
Research Category
Signaling
Research Sub Category
Metabolic Hormones & Receptors
Biochem/physiol Actions
This rabbit polyclonal antibody detects human Protein phosphatase 1 regulatory subunit 12A. It targets an epitope within 291 amino acids from the C-terminal region.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected MYPT1 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-386
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | chicken (immunogen homology) |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_990454 |
| UniProt accession no. | Q90623 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP1R12A(4659) |
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