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General description
Cyclin-dependent kinase 4 inhibitor B (UniProt: P55271; also known as p14-INK4b, p15-INK4b) is encoded by the Cdkn2b gene (Gene ID: 12579) in murine species. p15-INK4b is a ubiquitously expressed member of the INK4 family of cyclin-dependent kinase inhibitors. It induces a G1-phase cell cycle arrest through heterodimerization with cyclin-dependent kinase 4/6, thereby serves as a tumor suppressor. It also serves as a potential effector of TGF-beta induced cell cycle arrest. p15 also plays an important role in the regulation of cellular commitment of hematopoietic progenitor cells and myeloid cell differentiation. It contains four ankyrin repeats (aa 5-34; 38-66; 71-100; and 104-130). p15 shares extensive homology with p16 and serves an important backup function for p16 in senescence. Cdkn2b gene is frequently hypermethylated in myeloid neoplasia. Genes coding for both p15 and p16 are reported to often deleted in human tumors. p15 can serve as a good prognostic marker for monitoring the response to treatment with DNA methylation inhibitors. (Ref.: Wolff, L., and Bies, J. (2013). Blood Cells Mol. Dis. 50(4); 227-231).
~14 kDa observed; 13.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST/His-tagged recombinant full-length mouse Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).
Application
Research Category
Signaling
Anti-p15, clone PAT56B, Cat. No. MABS1950, is a rat monoclonal antibody that detects Cyclin-dependent kinase 4 inhibitor B (p15-INK4b) and has been tested for use in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected p15 in Immunohistochemistry applications (Munoz-Espin, D., et. al. (2013). Cell. 155(5):1104-18).
Biochem/physiol Actions
Clone PAT56B is a rat monoclonal antibody that specifically detects murine Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in NIH/3T3 cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected p15 in NIH/3T3 cell lysates.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | PAT56B, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_031696.1 |
| UniProt accession no. | P55271 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cdkn2B(12579) |
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¥ 2622.09
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¥ 1259.40
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¥ 8135.09
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