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General description
Nuclear receptor subfamily 5 group A member 2 (UniProt: O00482; also known as Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1) is encoded by the NR5A2 (also known as B1F, CPF, FTF) gene (Gene ID: 2494) in human. It is a member of the nuclear receptor superfamily that binds to the sequence element 5′-AACGACCGACCTTGAG-3′ of the enhancer II of hepatitis B virus genes. It contains a DNA binding region (aa 83-104) and two NR C4-type zinc finger domains (aa 86-106 and 122-146). It is abundantly expressed in pancreas, but at reduced levels in liver, and at very low levels in heart and lung. It s expression has been demonstrated in the Hep-G2 cell line. Four different isoforms of LRH-1 have been described that are generated by alternate splicing. LRH-1 is reported to be involved in the regulation of expression of transcription factors, such as Oct4, HNF1, 3, and 4, which are known to participate in embryonic development. Its presence in high levels in beta-III Tubulin+ and NeuN+ neurons of the mantle zone (MZ) in early neural tube of mice (E10.5 and E12.5) indicate its involvement in neural differentiation. (Ref.: Stergiopoulos, A., and Politis, PK (2016). Nat. Commun. DOI: 10.1038/ncomms12230; Venteclef, N et al. (2008). J. Biol. Chem. 283(7); 3694 3701).
~38 kDa observed; 38.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphorylated ser51 of human Eukaryotic translation initiation factor 2 subunit 1.
Application
Anti-phospho-eIF-2 (Ser51), Cat. No. 07-760-I, is a rabbit polyclonal antibody that detects Eukaryotic translation initiation factor 2 subunit 1 and has been tested for use in Peptide Inhibition Assay and Western Blotting.
Peptide Inhibition Assay: A 1:1,000 dilution from a representative lot detected phospho-eIF-2 (Ser51) in HeLa treated Calyculin A.
Research Category
Signaling
Biochem/physiol Actions
This rabbit polyclonal antibody detects elf-2 alpha phosphorylated on Serine 51 in human.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa treated with Calyculin A cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-eIF-2 (Ser51) in HeLa treated with Calyculin A cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Drosophila (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μL |
| manufacturer/tradename | Upstate® |
| technique(s) | ChIP: suitable (ChIP-seq),immunofluorescence: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_001285329 |
| UniProt accession no. | P41374 |
| shipped in | ambient |
| target post-translational modification | phosphorylation (pSer51) |
| Gene Information | Drosophila melanogaster ... Eif2Alpha(32617) |
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