Anti-VCAM-1 Antibody, clone M/K，500UG，CBL1300，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

Application

Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.
Studies of cell-cell interaction

Cell adhesion assays

Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures

Immunoprecipitation of the VCAM-1 integrin complex

Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.

Physical form

Format: Purified

Analysis Note

Control
EOMA and BMS2 endothelial cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB2242

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

技术参数 Specifications

biological source	rat
Quality Level	100
conjugate	unconjugated
antibody form	purified antibody
antibody product type	primary antibodies
clone	M/K - 2, monoclonal
species reactivity	mouse
manufacturer/tradename	Chemicon^®
technique(s)	flow cytometry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable
isotype	IgG1κ
NCBI accession no.	NM_001078.2,NM_080682.1
UniProt accession no.	P19320
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	human ... L1CAM(3897), VCAM1(7412)

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