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General description
This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in IF, IP, Neutralization Studies, Paraffin Sections, Immunoblotting for the detection of IGF-IR (Ab-1).
Immunogen
Human
Application
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Neutralization Studies (1 µg/ml; see application references)
Paraffin Sections (not recommended)
Immunoblotting (not recommended)
Physical form
Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
Preparation Note
Reconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
Other Notes
Blocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
Roth, R. 1988. Science239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.
Rosen, O.M. 1987. Science257, 1452.
Rechler, M.M., and Nissley, S.P. 1986. Hormone Res.24, 152.
Ullrich, A., et al. 1986. EMBO J.5, 2503.
Zapf, S., and Froesch, E.R. 1986. Hormone Res.24, 121.
Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
Kull, F.C., et al.1983. J. Biol. Chem.258, 6561.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | αIR3, monoclonal |
| form | lyophilized |
| does not contain | preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (Immunofluorescence (1-5 µg/mL) Immunoprecipitation (1 µg/sample) Neutralization Studies (1 µg/mL; Paraffin Sections (not recommended) Immunoblotting (not recommended)) |
| isotype | IgG1 |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... IGF1R(3480) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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