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General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.
~17 kDa
Application
ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Multiplexing:
This antibody specifically recognizes histone H3 phosphorylated on Ser10 by Luminex® assay.
Biochem/physiol Actions
Broad species cross-reactivity is expected, based on sequence homology.
Physical form
Format: Purified
Analysis Note
Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1093
Legal Information
Luminex is a registered trademark of Luminex Corp
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | CMA312, monoclonal |
| species reactivity | vertebrates, human |
| technique(s) | ChIP: suitable,ELISA: suitable,immunocytochemistry: suitable,multiplexing: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_003484.1 |
| UniProt accession no. | P84243 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer10) |
| Gene Information | human ... H3C1(8350) |
| 长度(mm) | |
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