- 商品介绍
- 规格参数
- 包装参数
Immunogen
Human recombinant tumor necrosis factor α.
Application
Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.
Immunohistochemistry detected TNF-α.
TNF-α Neutralization Assay
1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.
2. Incubate overnight at 37°C in humidified 5% CO2 incubator.
3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL
4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.
5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.
6. Incubate 24 hours at 37°C, 5% CO2.
7. Score wells for cytotoxicity.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.
Physical form
Format: Purified
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to one year.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1114
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| clone | 2C8, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,neutralization: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_000594.2 |
| UniProt accession no. | P01375 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TNF(7124) |
| 长度(mm) | |
| 宽度(mm) | |
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