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General description
Neuroendocrine convertase 2 (EC 3.4.21.94; UniProt P16519; also known as KEX2-like endoprotease 2, NEC 2, PC2, Prohormone convertase 2, Proprotein convertase 2) is encoded by the PCSK2 (also known as NEC2) gene (Gene ID 5126) in human. Neuroendocrine convertase 2/PC2 belongs to the family of subtilisin-related proteolytic enzymes involved in the proteolytic cleavages of peptide hormone precursors. For example, during the multistep processing of the peptide precursor proopiomelanocortin (POMC), PC2 catalyzes the cleavage of adrenocorticotropic hormone 1–39 (ACTH) into adrenocorticotropic hormone 1–14, which is then further processed to produce α-melanocyte-stimulating hormone (α-MSH). PC2 is produced initially in a prepro-form with a signal peptide (a.a. 1-25) and a propeptide (a.a. 26-109) sequence, the removal of which yields the mature 529-amino acid enzyme (a.a. 110-638). The neuroendocrine protein 7B2 blocks proPC2 from forming unactivatable aggregates and is absolutely required for processing proCP2 into active enzyme. Newly synthesized proPC2 is N-glycosylated in the ER and undergoes rate-limiting folding process before it can bind 7B2 and be transported to the TGN/ secretory granules for maturation by sulfation and proteolytic cleavage.
~68 kDa calculated. Target band appears larger than the calculated molecular weight of the mature form (58.28 kDa/mouse & 58.00 kDa/human) due to posttranslational glycosylation and sulfation. 67.97/63.72/68.12 kDa (human isoform 1/2/3 proforms), 70.57/66.31/68.58 kDa (human isoform 1/2/3 prepro-forms) calculated.
Immunogen
Epitope: Near the N-terminus of mature PC2.
GST-tagged recombinant human mature PC2 N-terminal fragment.
Application
Anti-Prohormone Convertase PC2 Antibody is an antibody against Prohormone Convertase PC2 for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Prohormone Convertase PC2 in human cerebral cortex, pancreas and testis tissue sections.
Research Category
Neuroscience
Research Sub Category
Hormones & Receptors
Biochem/physiol Actions
This polyclonal antibody detects the ~68 kDa secreted mature PC2 in mouse cerebellum lysate by Western blotting. Expected to recognize also the Prepro- and pre-forms of all three spliced human isoforms reported by UniProt (P16519).
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse cerebellum tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohormone Convertase PC2 in 10 µg of mouse cerebellum tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: AB15610
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_002585 |
| UniProt accession no. | P16519 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PCSK2(5126) |
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