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- 规格参数
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General description
Myosin is a hexameric protein consisting of two heavy chains including Myosin-2 (MYH2), two regulatory light chains, and two alkali light chains. The myosin-2 heavy chain interacts with the actin cytoskeleton and mediates muscle contractility in eukaryotic cells. Defects in myosin-2 result in inclusion body myopathy type 3.
~220 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to mouse Myosin 2.
Application
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Myosin 2 Antibody, clone 5B11.1 is validated for use in Western Blotting, IHC for the detection of Myosin 2.
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Myosin-2 in L6 cell lysate (Myosin may be observed as a smear in some cell lysates).
Western Blot Analysis: A representative lot detected Myosin-2 in mouse heart cell cytosolic extracts.
Physical form
Format: Purified
Purified mouse monoclonal IgG3κ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human cardiac muscle tissue
Evaluated by Immunohistochemistry in human cardiac muscle tissue.
Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected Myosin-2 in human cardiac muscle tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 5B11.1, monoclonal |
| species reactivity | mouse, human, rat |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG3κ |
| NCBI accession no. | NP_060004 |
| UniProt accession no. | Q9UKX2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MYH2(4620) |
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¥ 5167.58
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