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General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2. While lysine demethylation is mediated by enzymes such as JmjC domain-containing proteins.
~15 kDa observed. Molecular weight was observed in parent product MABE263
Immunogen
KLH-conjugated linear peptide corresponding to human unmethylated Histone H3 (Lys9).
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
See data sheet for Cat. No. MABE263, Anti-unmethylated Histone H3 (Lys9), clone 9B1-2G6, for additional Western blot and dot blot specificity analysis performed using an unconjugated version of this antibody.
This Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6 is validated for use in Immunocytochemistry for the detection of unmethylated Histone H3 (Lys9).
Biochem/physiol Actions
This antibody recognizes Histone H3 unmethylated at Lys9. This antibody does not cross react with methyl-Histone (Lys29). This antibody detects unmethylated Histone H3 (Lys9) when Ser10 is phosphorylated.
Physical form
Protein G Purified
Purified conjugated mouse monoclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Immunocytochemistry Analysis:
A 1:50 dilution of this antibody detected unmethylated Histone H3 (Lys9) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 300 |
| conjugate | ALEXA FLUOR™ 647 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 9B1-2G6, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NP_038578 |
| UniProt accession no. | P68431 |
| shipped in | wet ice |
| Gene Information | human ... HIST1H3F(8968) |
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