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General description
Native lactate dehydrogenase from rabbit muscle. Catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH 6.0.
Note: 1 KU = 1000 units.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Cahn, R.C., et al. 1962. Science136, 962.
One unit is defined as the amount of enzyme that will convert 1.0 µmol pyruvate to L-lactate per min in 100 mM phosphate buffer at 30°C, pH 7.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| description | Merck USA index - 14, 5334 |
| Quality Level | 100 |
| form | lyophilized |
| specific activity | ≥200 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| impurities | <0.005% GOT and GPT |
| solubility | low ionic strength buffer: soluble,water: soluble |
| storage temp. | −20°C |
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