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General description
Interleukin-2 receptor subunit alpha (UniProt: P01589; also known as IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25) is encoded by the IL2RA gene (Gene ID: 3559) in human. IL-2-RA is a non-covalent dimer of an alpha (CD25), beta unit (CD122). IL-2-RA is involved in the regulation of immune tolerance and controls the activity of T cells. IL-2RA can exist in three different forms; a high affinity dimer, an intermediate affinity monomer of beta subunit, and a low affinity monomer of alpha subunit. The high and intermediate affinity forms can also associate with a gamma subunit (CD132). CD25 is rapidly up-regulated upon activation of T cells. Activated T cells, regulatory T cells (Tregs) and NK cells express high levels of CD25, and high affinity expression of IL-2-RA is largely limited to these cells. CD25 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 22-240), a transmembrane domain (aa 241-250), and a short cytoplasmic tail (aa 260-272). It also contains two Sushi domains (aa 22-84 and 123-186). Mutations in IL2RA gene have been linked to insulin-dependent diabetes mellitus and immunodeficiency with lymphoproliferation that leads to recurrent viral, fungal, and bacterial infections. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. Ref.: (Driesen, J., et al. (2008). Immunobiology 213(9-10); 849-858).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
GST/His-tagged recombinant fragment corresponding to 119 amino acids from the N-terminal, extracellular domain of human IL-2 receptor subunit alpha (CD25).
Application
Anti-CD25 (IL2-RA), clone 1K6 ZooMAb, Cat. No. ZRB1412, is a recombinant Rabbit monoclonal antibody that targets CD25 (IL2-RA) and is tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD25 (IL2-RA) in human lymph node tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD25 (IL2-RA) in human tonsil tissue sections.
Flow Cytometry Analysis: 1μg from a representative lot detected CD25 (IL2-RA) in one million human peripheral blood mononuclear cells (PBMC).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1K6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects IL-2 receptor subunit alpha (CD25). It targets an epitope within 119 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 30.82 kDa,observed mol wt ~35 kDa |
| species reactivity | mouse, human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | flow cytometry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P01589 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... IL2RA(3559) mouse ... Il2Ra(16184) |
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