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General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Major envelope glycoprotein (UniProt: P17501) is encoded by the GP64 (also known as GP67, ORF128) gene (Gene ID: 1403961) in Autographa californica nuclear polyhedrosis virus (AcMNPV). The major envelope glycoprotein GP64 is a class III fusion protein primarily found in baculoviruses, such as the Autographa californica multiple nucleopolyhedrovirus (AcMNPV). GP64 plays a crucial role in viral entry by mediating low-pH-triggered membrane fusion between the viral envelope and host endosomal membranes. Structurally, GP64 undergoes significant conformational changes in response to acidic conditions, transitioning from a pre-fusion state, dominated by β-sheets and a coiled-coil architecture, to a post fusion conformation essential for membrane fusion events. GP64 is indispensable for virion assembly and budding, particularly in the budded virus (BV) phenotype, which facilitates systemic infection within insect hosts. The protein also facilitates efficient intracellular trafficking to the plasma membrane, where it assembles into budding virions. This trafficking involves critical interactions with host proteins such as Rab GTPases (Rab1, Rab4), clathrin heavy chain, and adaptors like AP-1-2β and Snap29, highlighting its integration into vesicular transport pathways. Expression studies reveal that GP64 is localized predominantly at the plasma membrane during viral egress, although its synthesis commences in the endoplasmic reticulum (ER). Notably, the GP64 signal peptide has been shown to activate the ER-associated degradation (ERAD) pathway, which underscores its regulatory role in protein quality control during expression. GP64 has applications beyond baculovirus biology. It is integral to the Baculovirus Expression Vector System (BEVS), a widely utilized platform for recombinant protein production in biomedical research. modified GP64 proteins are being explored for medical applications, such as peptide display for vaccine development. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Dong S., and Blissard G.W., (2012), J Virol, 86(18), 9617-9628; Rodríguez-Hernández A.P., et al. (2023). Viruses. 15(2), 411; Hodgson J.J, et al., (2022). J Virol.;96(12), e0021522.)
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminal half of human AcV5.
Application
Quality Control Testing
Evaluated by Western Blotting with His-tagged recombinant AcMNPV-gp64 protein (AcV5).
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected a His-tagged recombinant AcMNPV-gp64 protein (AcV5).
Tested Applications
Enzyme Immunoassay (ELISA): A representative lot of this antibody detected recombinant AcMNPV-gp64 protein in ELISA application.
Affinity Binding Assay: A representative lot of this antibody bound with AcV5 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 3I3 is a ZooMAb® rabbit recombinant monoclonal antibody that detects AcV5. It targets an epitope within 9 amino acids from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3I3, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 58.57 kDa,observed mol wt ~60 kDa |
| purified by | using Protein A |
| species reactivity | virus |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_054158 |
| UniProt accession no. | P17501 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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