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General description
Transient receptor potential cation channel subfamily V member 6 (UniProt Q9H1D0; also known as Alu-binding protein with zinc finger domain, Calcium transport protein 1, Calcium transporter-like protein, CaT-L, CaT-like, CaT1, ECaC2, Epithelial apical membrane calcium transporter/channel CaT1, Epithelial calcium channel 2, TrpV6) is encoded by the TRPV6 (also known as ABP/ZF, CAT1, CATL, ECAC2, ZFAB) gene (Gene ID 55503) in human. TRPV6 belongs to the transient receptor potential (TRP) family of membrane proteins. The human TRPV6 is an 765-amino acid, six-transmembrane protein with both N- and C-termini exposed intracellularly. TRPV6 contains a pore-forming loop domain located between the fifth and sixth transmembrane region. Among all the TRPV family members, TRPV5 and TRPV6 are the two highly Ca2+-selective channels that play pivotal roles in the Ca2+-reabsorption in the kidney and intestine, respectively. TRPV6 is found in the apical brush-border membrane of the intestinal enterocytes where it regulates calcium entry into the cells. TRPV6 expression is also reported in human placenta, pancreas, and prostate gland. Vitamin D treatment of human colon cancer Caco-2 cells and duodenal explants results in upregulated TRPV6 mRNA levels.
~70 kDa observed.
Immunogen
Epitope: C-terminal region.
GST-tagged recombinant human TrpV6 C-terminal fragment.
Application
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Western Blotting Analysis: A 1:500 dilution from a representative lot detected TrpV6 in 10 µg of LNCaP prostate adenocarcinoma and K562 erythroleukemia cell lysates.
Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected TrpV6 in human pancreas, human small intestine, rat colon tissue, and mouse brain tissues.
Biochem/physiol Actions
Expected to react with both spliced isoforms of human TrpV6 reported by UniProt (Q9H1D0).
Physical form
In culture supernatant without preservatives.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in Y79 retinoblastoma cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TrpV6 in 10 µg of Y79 retinoblastoma cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | 4A5.1, monoclonal |
| species reactivity | mouse, human, rat |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_061116 |
| UniProt accession no. | Q9H1D0 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | rat ... Trpv6(114246) |
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