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General description
Protein Wnt-10b (Wnt-10b) is a member of the Wnt family that plays an important role in normal development such as regulation of cell fate, patterning during embryogenesis, and neoplastic transformation. Wnt-10b may promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt-10b has multiple roles in cell growth and differentiation. Mutations in Wnt-10b have been associated with an autosomal receesive disorder known as split-hand/foot malformation type 6 (SHFM6).
~45 kDa observed. Uncharacterized bands may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human Wnt-10b.
Application
Detect Wnt using this rabbit polyclonal antibody, Anti-Wnt Antibody-10b validated for use in western blotting & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected Wnt-10b in human skeletal muscle tissue.
Analysis Note
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected Wnt-10b in 15 µg of human skeletal muscle tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| UniProt accession no. | O00744 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... WNT10B(7480) |
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